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R&D Systems cxcl10 concentration
Cxcl10 Concentration, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cxcl10 concentration/product/R&D Systems
Average 96 stars, based on 157 article reviews

cxcl10 concentration - by Bioz Stars, 2026-06

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The viperin secretome and CXCL10. At day 5 in ATDC5 chondrogenic differentiation, viperin expression was reduced by either transfection of a viperin-specific siRNA duplex (Viperin siRNA) or a scrambled control siRNA duplex (Scrambled siRNA); or viperin expression was further induced by transfection of a p3xFLAG-viperin plasmid (FLAG-Viperin) or the empty p3xFLAG plasmid (FLAG-empty). The cells were then further differentiated until day 7, and viperin expression levels were determined ( A and B ). Immunoblots are shown for two independent representative examples. Culture supernatants from these day 7 cultures were collected, and the protein secretome was determined by label-free LC-MS/MS. Differentially secreted extracellular protein species (control versus condition) with p < 0.05 are shown ( C ). FC , fold change. In culture supernatants analyzed with LC-MS/MS, secreted CXCL10 levels were determined ( D ). In ATDC5 culture supernatants from , secreted CXCL10 levels were determined ( E ). The p values are indicated, and error bars indicate means ± S.E.

Journal: The Journal of Biological Chemistry

Article Title: The antiviral protein viperin regulates chondrogenic differentiation via CXCL10 protein secretion

doi: 10.1074/jbc.RA119.007356

Figure Lengend Snippet: The viperin secretome and CXCL10. At day 5 in ATDC5 chondrogenic differentiation, viperin expression was reduced by either transfection of a viperin-specific siRNA duplex (Viperin siRNA) or a scrambled control siRNA duplex (Scrambled siRNA); or viperin expression was further induced by transfection of a p3xFLAG-viperin plasmid (FLAG-Viperin) or the empty p3xFLAG plasmid (FLAG-empty). The cells were then further differentiated until day 7, and viperin expression levels were determined ( A and B ). Immunoblots are shown for two independent representative examples. Culture supernatants from these day 7 cultures were collected, and the protein secretome was determined by label-free LC-MS/MS. Differentially secreted extracellular protein species (control versus condition) with p < 0.05 are shown ( C ). FC , fold change. In culture supernatants analyzed with LC-MS/MS, secreted CXCL10 levels were determined ( D ). In ATDC5 culture supernatants from , secreted CXCL10 levels were determined ( E ). The p values are indicated, and error bars indicate means ± S.E.

Article Snippet: To determine CXCL10 protein concentrations in media, the mouse CXCL10/IP-10/CRG-2 DuoSet ELISA (R&D catalog no. DY466) and the human CXCL10/IP-10 Duoset ELISA (R&D catalog no. DY266) were utilized according to the manufacturer's protocol.

Techniques: Expressing, Transfection, Control, Plasmid Preparation, Western Blot, Liquid Chromatography with Mass Spectroscopy

CXCL10 attenuates chondrogenic differentiation. ATDC5 cells were differentiated and exposed to mCXCL10 from day 5 until day 7. RNA was isolated, and expression of Sox9, Col2a1, Runx2, Col10a1, and Alpl mRNAs was determined ( A ). hBMSCs were differentiated in the chondrogenic lineage for 7 days and exposed to hCXCL10 until day 21. RNA was isolated, and expression of COL2A1 and COL10A1 mRNAs was determined ( B ). ATDC5 data were acquired from four biological replicates and normalized to β-actin mRNA levels, and individual normalized values are presented in dot plots. hBMSC gene expression data were normalized to CYCLOPHILIN mRNA levels. Individual data points in dot plots represent the average values of four biological replicates of one hBMSC donor. For statistical evaluation, an independent samples t test was performed relative to the corresponding controls using GraphPad Prism 5. The p values are indicated, and error bars show means ± S.E. The graphs show representative examples of three individual experiments.

Journal: The Journal of Biological Chemistry

Article Title: The antiviral protein viperin regulates chondrogenic differentiation via CXCL10 protein secretion

doi: 10.1074/jbc.RA119.007356

Figure Lengend Snippet: CXCL10 attenuates chondrogenic differentiation. ATDC5 cells were differentiated and exposed to mCXCL10 from day 5 until day 7. RNA was isolated, and expression of Sox9, Col2a1, Runx2, Col10a1, and Alpl mRNAs was determined ( A ). hBMSCs were differentiated in the chondrogenic lineage for 7 days and exposed to hCXCL10 until day 21. RNA was isolated, and expression of COL2A1 and COL10A1 mRNAs was determined ( B ). ATDC5 data were acquired from four biological replicates and normalized to β-actin mRNA levels, and individual normalized values are presented in dot plots. hBMSC gene expression data were normalized to CYCLOPHILIN mRNA levels. Individual data points in dot plots represent the average values of four biological replicates of one hBMSC donor. For statistical evaluation, an independent samples t test was performed relative to the corresponding controls using GraphPad Prism 5. The p values are indicated, and error bars show means ± S.E. The graphs show representative examples of three individual experiments.

Techniques: Isolation, Expressing, Gene Expression

TGF-β/SMAD2/3 activity is controlled by the viperin secretome and CXCL10. At day 5 in ATDC5 chondrogenic differentiation, viperin expression was either reduced by transfection of a viperin-specific siRNA duplex (Viperin siRNA) or a scrambled control siRNA duplex (Scrambled siRNA) or further induced by transfection of a p3xFLAG-viperin plasmid (FLAG-Viperin) or the empty p3xFLAG plasmid (FLAG-empty). The cells were then further differentiated until day 7, and viperin expression levels were determined ( A ). Conditioned culture supernatants (CM) from these day 7 cultures were collected. Proliferating ATDC5 were used as a TGF-β/SMAD2/3 bioassay by co-transfecting a CAGA-12 firefly luciferase TGF-β/SMAD3 reporter and pGluc-CMV plasmid. The TGF-β/SMAD2/3 bioassay was then exposed to the CM supernatants for 24 h, and supernatant and cells were collected for bioluminescence analyses. Firefly bioluminescence was normalized to the Gaussia signal ( B ). Gene expression of downstream TGF-β target genes Pai1 and Smad7 was determined on samples from B /C ( C and D ). ATDC5 cells were differentiated and co-transfected with a CAGA-12 firefly luciferase TGF-β/SMAD3 reporter and pGluc-CMV plasmid on day 5 in chondrogenic differentiation. At day 6 in differentiation, the cells were exposed to 0.5, 5, 50, or 200 ng/ml recombinant mouse CXCL10 for 24 h until day 7. Culture supernatant and cells were collected for bioluminescence analyses. Firefly bioluminescence was normalized to the Gaussia signal ( E ). ATDC5 cells were differentiated until day 5 and then exposed to 0.5, 5, 50, or 200 ng/ml mouse CXCL10 until day 7. Protein extracts were separated by SDS-PAGE and electroblotted on membranes, followed by pSMAD2C immunodetection. GAPDH was used as a loading control ( F ). All quantitative data were acquired from four biological replicates. RT-qPCR data were normalized to β-actin mRNA levels, and individual normalized values are presented in dot plots. Bioluminescence data are presented as normalized RLUs. RLUs of controls were set at 1, and RLUs of conditions were calculated relative to the control RLUs. For statistical evaluation, an independent samples t test was performed relative to the corresponding controls using GraphPad Prism 5. The p values are indicated, and error bars represent means ± S.E. Graphs are representative examples of three individual experiments.

Journal: The Journal of Biological Chemistry

Article Title: The antiviral protein viperin regulates chondrogenic differentiation via CXCL10 protein secretion

doi: 10.1074/jbc.RA119.007356

Figure Lengend Snippet: TGF-β/SMAD2/3 activity is controlled by the viperin secretome and CXCL10. At day 5 in ATDC5 chondrogenic differentiation, viperin expression was either reduced by transfection of a viperin-specific siRNA duplex (Viperin siRNA) or a scrambled control siRNA duplex (Scrambled siRNA) or further induced by transfection of a p3xFLAG-viperin plasmid (FLAG-Viperin) or the empty p3xFLAG plasmid (FLAG-empty). The cells were then further differentiated until day 7, and viperin expression levels were determined ( A ). Conditioned culture supernatants (CM) from these day 7 cultures were collected. Proliferating ATDC5 were used as a TGF-β/SMAD2/3 bioassay by co-transfecting a CAGA-12 firefly luciferase TGF-β/SMAD3 reporter and pGluc-CMV plasmid. The TGF-β/SMAD2/3 bioassay was then exposed to the CM supernatants for 24 h, and supernatant and cells were collected for bioluminescence analyses. Firefly bioluminescence was normalized to the Gaussia signal ( B ). Gene expression of downstream TGF-β target genes Pai1 and Smad7 was determined on samples from B /C ( C and D ). ATDC5 cells were differentiated and co-transfected with a CAGA-12 firefly luciferase TGF-β/SMAD3 reporter and pGluc-CMV plasmid on day 5 in chondrogenic differentiation. At day 6 in differentiation, the cells were exposed to 0.5, 5, 50, or 200 ng/ml recombinant mouse CXCL10 for 24 h until day 7. Culture supernatant and cells were collected for bioluminescence analyses. Firefly bioluminescence was normalized to the Gaussia signal ( E ). ATDC5 cells were differentiated until day 5 and then exposed to 0.5, 5, 50, or 200 ng/ml mouse CXCL10 until day 7. Protein extracts were separated by SDS-PAGE and electroblotted on membranes, followed by pSMAD2C immunodetection. GAPDH was used as a loading control ( F ). All quantitative data were acquired from four biological replicates. RT-qPCR data were normalized to β-actin mRNA levels, and individual normalized values are presented in dot plots. Bioluminescence data are presented as normalized RLUs. RLUs of controls were set at 1, and RLUs of conditions were calculated relative to the control RLUs. For statistical evaluation, an independent samples t test was performed relative to the corresponding controls using GraphPad Prism 5. The p values are indicated, and error bars represent means ± S.E. Graphs are representative examples of three individual experiments.

Techniques: Activity Assay, Expressing, Transfection, Control, Plasmid Preparation, Bioassay, Luciferase, Gene Expression, Recombinant, SDS Page, Immunodetection, Quantitative RT-PCR

The viperin–CXCL10–TGF-β/SMAD2/3 axis is deregulated in chondrocytic CHH cells. Rmrp RNA expression was reduced in ATDC5 cells by transfection of a specific siRNA duplex on day −1, day 2, and day 5 during chondrogenic differentiation. A scrambled siRNA was used as control. Samples were harvested for RT-qPCR analysis at day 0, 7, or 10 in differentiation. Expression of Rmrp ( A ) and viperin ( B ) was determined at indicated time points. The data were normalized to β-actin mRNA levels, and individual normalized values are presented in dot plots. The data were acquired from three biological replicates. An independent samples t test was performed relative to scrambled control using GraphPad Prism 5. The p values are indicated, and error bars represent means ± S.E. Human dermal fibroblasts from three CHH patients (RMRP alleles of CHH patients carried following mutations: 127G → A and 261 C → G; 4 C → T and 77C → T; 70 A → G and 70A → G), and three healthy controls were transdifferentiated into the chondrogenic lineage by hyperconfluent plating in wells coated with Aggrecan ( , ). RNA was isolated at day 3 of transdifferentiation, and gene expression of RMRP, SOX9, COL2A1, RUNX2, COL10A1, and ALPL mRNAs was determined ( C ). Gene expression of VIPERIN ( D ) and of PAI1 and SMAD7 ( F ) was determined in samples from C . Supernatants were collected from these cultures, and secreted CXCL10 protein was determined with ELISA ( E ). Gene expression data from transdifferentiated fibroblasts was normalized to CYCLOPHILIN mRNA levels, and individual normalized values are presented in dot plots. Secreted CXCL10 data are absolute concentrations (pg/ml) and presented in dot plots. For statistical evaluation, independent sample t tests were performed relative to healthy controls using GraphPad Prism 5. The p values are indicated. Error bars represent the means ± S.E. Graphs are representative examples of three independent experiments.

Journal: The Journal of Biological Chemistry

Article Title: The antiviral protein viperin regulates chondrogenic differentiation via CXCL10 protein secretion

doi: 10.1074/jbc.RA119.007356

Figure Lengend Snippet: The viperin–CXCL10–TGF-β/SMAD2/3 axis is deregulated in chondrocytic CHH cells. Rmrp RNA expression was reduced in ATDC5 cells by transfection of a specific siRNA duplex on day −1, day 2, and day 5 during chondrogenic differentiation. A scrambled siRNA was used as control. Samples were harvested for RT-qPCR analysis at day 0, 7, or 10 in differentiation. Expression of Rmrp ( A ) and viperin ( B ) was determined at indicated time points. The data were normalized to β-actin mRNA levels, and individual normalized values are presented in dot plots. The data were acquired from three biological replicates. An independent samples t test was performed relative to scrambled control using GraphPad Prism 5. The p values are indicated, and error bars represent means ± S.E. Human dermal fibroblasts from three CHH patients (RMRP alleles of CHH patients carried following mutations: 127G → A and 261 C → G; 4 C → T and 77C → T; 70 A → G and 70A → G), and three healthy controls were transdifferentiated into the chondrogenic lineage by hyperconfluent plating in wells coated with Aggrecan ( , ). RNA was isolated at day 3 of transdifferentiation, and gene expression of RMRP, SOX9, COL2A1, RUNX2, COL10A1, and ALPL mRNAs was determined ( C ). Gene expression of VIPERIN ( D ) and of PAI1 and SMAD7 ( F ) was determined in samples from C . Supernatants were collected from these cultures, and secreted CXCL10 protein was determined with ELISA ( E ). Gene expression data from transdifferentiated fibroblasts was normalized to CYCLOPHILIN mRNA levels, and individual normalized values are presented in dot plots. Secreted CXCL10 data are absolute concentrations (pg/ml) and presented in dot plots. For statistical evaluation, independent sample t tests were performed relative to healthy controls using GraphPad Prism 5. The p values are indicated. Error bars represent the means ± S.E. Graphs are representative examples of three independent experiments.

Techniques: RNA Expression, Transfection, Control, Quantitative RT-PCR, Expressing, Isolation, Gene Expression, Enzyme-linked Immunosorbent Assay

Model of the interactions between viperin and chondrogenic differentiation. A schematic model of the interactions between viperin and chondrogenic differentiation, suggested by our results. Viperin regulates protein secretion and controls the level of secreted CXCL10. CXCL10 inhibits TGF-β/SMAD2/3 activity, which in turn controls the level of chondrogenic differentiation.

Journal: The Journal of Biological Chemistry

Article Title: The antiviral protein viperin regulates chondrogenic differentiation via CXCL10 protein secretion

doi: 10.1074/jbc.RA119.007356

Figure Lengend Snippet: Model of the interactions between viperin and chondrogenic differentiation. A schematic model of the interactions between viperin and chondrogenic differentiation, suggested by our results. Viperin regulates protein secretion and controls the level of secreted CXCL10. CXCL10 inhibits TGF-β/SMAD2/3 activity, which in turn controls the level of chondrogenic differentiation.

Techniques: Activity Assay

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